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The Domain Name System (DNS) is a critical piece of Internet infrastructure with remarkably complex properties and uses, and accordingly has been extensively studied. In this study we contribute to that body of work by organizing and analyzing records maintained within the DNS as a bipartite graph. We find that relating names and addresses in this way uncovers a surprisingly rich structure. In order to characterize that structure, we introduce a new graph decomposition for DNS name-to-IP mappings, which we term elemental decomposition. In particular, we argue that (approximately) decomposing this graph into bicliques — maximally connected components — exposes this rich structure. We utilize large-scale censuses of the DNS to investigate the characteristics of the resulting decomposition, and illustrate how the exposed structure sheds new light on a number of questions about how the DNS is used in practice and suggests several new directions for future research. 

Abstract An athermal approach to mRNA enrichment from total RNA using a self‐immolative thioester linked nucleic acids (TENA) is described. Oligo(thymine) (oT) TENA has a six‐atom spacing between bases which allowed TENA to selectively base‐pair with polyadenine RNA. As a result of the neutral backbone of TENA and the hydrophobicity of the octanethiol end group, oT TENA is water insoluble and efficiently pulled down 93±2 % of EGFP mRNA at a concentration of 10 ng μL⁻¹. Self‐immolative degradation of TENA upon ambient temperature exposure to nucleophilic buffer components (Tris, DTT) allowed recovery of 55±27 ng of mRNA from 3.1 μg of total RNA, which was not statistically different from the amount recovered using Dynabeads® mRNA DIRECT Kit (89±24 ng). Gene expression as measured by RT‐qPCR was comparable for both enrichment methods, suggesting that the mild conditions required for enrichment of mRNA using oT TENA are compatible with RT‐qPCR and other downstream molecular biology applications.